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电针预处理对创伤后应激大鼠模型焦虑样 行为及前额叶皮质内源性大麻素相关基因 表达的影响
陈怡环薛芬顾婷婷王世全王化宁彭正午
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目的 观察电针(EA)预处理在预防创伤后应激障碍中的可能作用及其潜在的分子机制。 方法 将32 只健康雄性的SD 大鼠经1 周环境适应后,随机分为对照组(Sham组)、模型组(ESPS 组)、对 照电针组(Sham+EA 组)和模型电针组(ESPS+EA 组),每组8 只。Sham+EA 组和ESPS+EA 组的大鼠每天 接受电针刺激(百会穴,1 mA,2/15 Hz)30 min,连续1 周。另外两组给予假刺激(无电流)每天30 min,连 续1 周。然后对ESPS 组和ESPS+EA 组大鼠进行增强型单次延长应激(ESPS)造模处理。造模结束2 周 后,采用旷场实验和高架十字实验观察各组大鼠的行为,随后处死大鼠,用蛋白质印迹法检测各组大鼠 前额叶皮质内源性大麻素受体1(CB1R)、单酰基甘油脂肪酶(MAGL)和二酰基甘油脂肪酶(DAGLα)蛋 白的表达。结果 (1)ESPS处理导致大鼠产生焦虑样的行为,进入旷场中心区的时间和次数减少,进入 高架十字开臂的次数减少。ESPS 组大鼠前额叶皮质CB1R 和DAGLα蛋白表达下降,而MAGL的表达无 明显变化。(2)电针预处理可以改善大鼠的焦虑样行为,恢复ESPS模型大鼠前额叶皮质CB1R和DAGLα 的表达。结论 电针预处理减轻了ESPS诱导的大鼠焦虑样的行为,这可能与大脑前额叶皮层CB1R和 DAGLα的表达增加有关,但还需要进一步的研究来明确它们之间的关系。
基金项目:国家自然科学基金项目(81571309)
Effect of electroacupuncture pretreatment on anxiety-like behavior and expression of endogenous cannabinoidrelated genes in the prefrontal cortex of PTSD rats mode
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Abstract:
Objectives To observe the effect of Electroacupuncture( EA) pretreatment in the prevention of post-traumatic stress disorder( PTSD) and its underlying molecular mechanisms. Methods A total of 32 healthy male Sprague Dawley( SD) rats were randomly divided into four groups after adjusting the environment CB1R and DAGLα in the prefrontal cortex of ESPS model rats. Conclusions Electroacupuncture pretreatment alleviated ESPS-induced anxiety-like behavior in rats, which may be related to increased expression of CB1R and DAGLα in the prefrontal cortex of the brain. Further studies are needed to clarify their relevance. for 1 week: Sham group, ESPS group, Sham+EA group and ESPS+EA group, with 8 rats in each group. Rats in Sham+EA group and ESPS+EA group received electroacupuncture stimulation( Baihui, 1 mA, 2/15 Hz) for 30 minutes every day for 1 week. The other two groups received sham stimulation( EA treatment without electricity) for 1 week. Rats in ESPS group and ESPS+EA group were then exposed to enhanced single prolonged stress( ESPS). Two weeks after ESPS, the behavior of each rat was observed in the open field test and elevatedplus maze test. The expression of endocannabinoid receptor type 1( CB1R), monoacylglycerol lipase( MAGL) and diacylglycerol lipase( DAGLα) were measured by Western blot. Results (1) ESPS treatment resulted in anxiety-like behavior in rats, reduced time and frequency of entering the open-field central area, and reduced frequency of entering the elevated cross arm. The expression levels of CB1R and DAGLα in the prefrontal cortex of rats in the ESPS group decreased, while the expression of MAGL did not change significantly. (2)Electroacupuncture pretreatment can ameliorate the anxiety-like behavior, and restore the expression of

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